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TLS Online TPP Program

#Question id: 12349

Males in a population differ in the time they spend displaying to females. A researcher hypothesizes that predators are responsible for these differences. Males display for longer durations when there are no predators in the vicinity and for shorter durations when there is a predator nearby. Which of the following study designs is the most appropriate test of this hypothesis?

#Unit 10. Ecological Principles
  1. Map predator distribution in the area; measure the abundance of females; quantify the natural variation in male display rates in areas without predators
  2. Measure display  rates of  males at  the beginning  of  the breeding season; remove all  predators from the study site; then measure male display rates later in the breeding season; repeat for multiple populations
  3. Measure display rates of males in areas with and without predators; randomly assign males to two treatments: (i) capture and release back in original area (ii) capture and switch between areas; measure display rates for all experimental males
  4. Measure display rates of males in areas with and without predators; randomly assign males to two treatments: (i) addition of females to the area (ii) removal of females from the area; measure display rates for all experimental males
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TLS Online TPP Program

#Question id: 23181

#Unit 13. Methods in Biology

__________ type of biosensors exist to measure the concentration and location of Ca2+ and the activation state of GTPase switch proteins.
TLS Online TPP Program

#Question id: 23180

#Unit 13. Methods in Biology

FRET is very sensitive to small changes in distance and in practice is not detectable at distances greater than 
TLS Online TPP Program

#Question id: 23179

#Unit 13. Methods in Biology

In order to observe FRET two flurophores must be in close proximity of about 10nm distance. Given below are few flurorescent molecules.
1. YFP
2. RFP
3. GFP
4. CFP
Which option below contains correct combination of flurorescent molecules that can give FRET?
TLS Online TPP Program

#Question id: 23173

#Unit 13. Methods in Biology

There are various drawbacks still in fluorescence microscopy such as
1.  Light emitted comes from molecules above and below the plane of focus
2. Blurred image due to superposition of fluorescent images
Which of the above statement is/ are correct?
TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?
1.  It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?