In some cells, there are many ion electrochemical gradients across the plasma membrane even though there are usually only one or two proton pumps present in the membrane. The gradients of the other ions are most likely accounted for by:

TLS Online TPP Program

#Question id: 1770

#SCPH05 I Biotechnology

Which of the following immunoglobulin has the highest avidity of the immunoglobulins?

TLS Online TPP Program

#Question id: 13090

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 3’s plasmid is;

TLS Online TPP Program

#Question id: 10564

#SCPH06 I Botany

The term Ys , called the solute potential or the osmotic potential, represents the effect of dissolved solutes on water potential,

a) The mixing of solutes and water reduces the disorder of the system and thereby lowers free energy

b) Solutes reduce the free energy of water by diluting the water

c) The mixing of solutes and water increases the disorder of the system and thereby lowers free energy

d) Solutes increases the free energy of water by diluting the water

 Which one of the following statements are INCORRECT?

TLS Online TPP Program

#Question id: 51

#I Life Science/ Life Sciences Group – I-V

 If the equilibrium constant, Keq, for the reaction ATP → ADP + Pi is 2.22 × 10⁵ M, calculate the standard free-energy change, ΔG°, for the synthesis of ATP from ADP and Pi at 25 °C.

TLS Online TPP Program

#Question id: 14501

#SCPH01 Biochemistry

In a population having 50% rice consumers, what is the probability that three or less out of 10 are rice consumers?