Following statements are regarding to the spindle position checkpoint pathway in budding yeast.

A. During interphase and early mitosis, Cdc14 is sequestered and inactivated in the nucleolus. Inactive Tem1-GDP associates with the spindle pole body (SPB) nearest to the bud as soon as the mitotic spindle forms.

B. If chromosome segregation occurs properly, extension of the spindle microtubules inserts the daughter SPB into the bud, causing Tem1 to be activated by an unknown mechanism.

C. Tem1-GDP activates a protein kinase cascade, which then promotes the release of active Cdc14 from the nucleolus and exit from mitosis.

D. If the spindle apparatus fails to place the daughter SPB in the bud, Kin4 (cyan), an inhibitor of Tem1, is recruited from the parent cell cortex to the parent-cell-located SPB and maintains Tem1 in the GTP-bound form, and mitotic exit does not occur.

Which of the following are correct?

TLS Online TPP Program

#Question id: 15699

#SCPH05 I Biotechnology

The PROSITE pattern representing the conserved sequence motif for a new family of AMP binding protein is [LIVMFY]-X(2)-[STG]-[STAG]-G-[ST]. You are given a sequence of a 15-amino acid stretch starting from the first residue of the motif. Which one of the following proteins is likely to have AMP binding function?

TLS Online TPP Program

#Question id: 15699

#SCPH05 I Biotechnology

The PROSITE pattern representing the conserved sequence motif for a new family of AMP binding protein is [LIVMFY]-X(2)-[STG]-[STAG]-G-[ST]. You are given a sequence of a 15-amino acid stretch starting from the first residue of the motif. Which one of the following proteins is likely to have AMP binding function?

TLS Online TPP Program

#Question id: 15700

#SCPH01 Biochemistry

In a sequence database of a given size, which of the following expressions is likely to retrieve more matches (X means any amino acid; any of the residues in square brackets can occupy that position)?

TLS Online TPP Program

#Question id: 15700

#I Life Science/ Life Sciences Group – I-V

In a sequence database of a given size, which of the following expressions is likely to retrieve more matches (X means any amino acid; any of the residues in square brackets can occupy that position)?

TLS Online TPP Program

#Question id: 15700

#SCPH05 I Biotechnology

In a sequence database of a given size, which of the following expressions is likely to retrieve more matches (X means any amino acid; any of the residues in square brackets can occupy that position)?

TLS Online TPP Program

#Question id: 15700

#SCPH05 I Biotechnology

In a sequence database of a given size, which of the following expressions is likely to retrieve more matches (X means any amino acid; any of the residues in square brackets can occupy that position)?