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TLS Online TPP Program
#Question id: 13057
#SCPH05 I Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?
TLS Online TPP Program
#Question id: 8912
#SCPH06 I Botany
Both animals and fungi are heterotrophic. What distinguishes animal heterotrophy from fungal heterotrophy is that only animals derive their nutrition
TLS Online TPP Program
#Question id: 920
#I Life Science/ Life Sciences Group – I-V
Which of these is able to cross the inner mitochondrial membrane?
TLS Online TPP Program
#Question id: 1568
#SCPH01 Biochemistry
which one of these is not a characteristics of B-cell?
TLS Online TPP Program
#Question id: 17276
#SCPH05 I Biotechnology
The viscosity of water at STP is approximately