Which bacterial strain was improved by scientists to be able to degrade 2,4-dinitrotoluene and promote plant growth

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#Question id: 335

#SCPH05 I Biotechnology

Suppose the major solutes in intact lysosomes are KCl (~0.1 M) and NaCl (~0.03 M). When isolating lysosomes, what concentration of sucrose is required in the extracting solution at room temperature (25 °C) to prevent swelling and lysis?

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#Question id: 15231

#SCPH06 I Botany

Which of the following is true for Capillary electrophoresis (CE) resolution

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#Question id: 4201

#SCPH28 | Zoology

The role of GTP in the translocation step of protein synthesis is to supply energy for

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#Question id: 19919

#SCPH01 Biochemistry

Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. Which plots correctly verify the Scatchard equation that determined by equilibrium dialysis?

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#Question id: 13088

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;