Which of the following statements is TRUE of C3b?

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#Question id: 19212

#SCPH28 | Zoology

What actually happens in catalytic biosensors?

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#Question id: 555

#SCPH01 Biochemistry

The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by To determine Km from a double-reciprocal plot, you would:

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#Question id: 12106

#SCPH28 | Zoology

Which of the following best describes resource partitioning?

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#Question id: 10514

#SCPH05 I Biotechnology

Volicitin was identified as the active substance in the form of

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#Question id: 13054

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?