Fusicoccin is a nonspecific toxin from the fungus Fusicoccum amygdali function as,

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#Question id: 15637

#SCPH01 Biochemistry

You have isolated two mutations in the Lac operon that cause constitutive expression of Lac genes. You designate these mutants Lac1– and Lac2–. Making use of an F' that carries the Lac operon with the LacY gene mutated, you construct strains that you test for both ß-galactosidase activity and Lac permease activity with results shown below.

     

Classify each mutation as dominant or recessive and as cis- or trans-acting, giving the experimental result that allows you to arrive at each conclusion. Finally, deduce what type of Lac mutation best fits the properties of Lac 1– and of Lac 2–.

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#Question id: 15637

#SCPH06 I Botany

You have isolated two mutations in the Lac operon that cause constitutive expression of Lac genes. You designate these mutants Lac1– and Lac2–. Making use of an F' that carries the Lac operon with the LacY gene mutated, you construct strains that you test for both ß-galactosidase activity and Lac permease activity with results shown below.

     

Classify each mutation as dominant or recessive and as cis- or trans-acting, giving the experimental result that allows you to arrive at each conclusion. Finally, deduce what type of Lac mutation best fits the properties of Lac 1– and of Lac 2–.

TLS Online TPP Program

#Question id: 15637

#SCPH28 | Zoology

You have isolated two mutations in the Lac operon that cause constitutive expression of Lac genes. You designate these mutants Lac1– and Lac2–. Making use of an F' that carries the Lac operon with the LacY gene mutated, you construct strains that you test for both ß-galactosidase activity and Lac permease activity with results shown below.

     

Classify each mutation as dominant or recessive and as cis- or trans-acting, giving the experimental result that allows you to arrive at each conclusion. Finally, deduce what type of Lac mutation best fits the properties of Lac 1– and of Lac 2–.

TLS Online TPP Program

#Question id: 15638

#SCPH01 Biochemistry

You have isolated two mutations that show decreased expression of the Lac operon. However, unlike like the promoter mutations, these mutations don’t respond to the inducer IPTG. These mutations, designated Lac3– and Lac4–, are evaluated for the quantity of ß-galactosidase and permease activity expressed with or without IPTG:

 

Mapping experiments reveal that Lac3– and Lac4– are different short deletions located in the region before the start of the LacZ gene. Given the data shown above suggest which genetic element(s) in addition to part of the promoter has been deleted in each mutant. 

TLS Online TPP Program

#Question id: 15638

#SCPH06 I Botany

You have isolated two mutations that show decreased expression of the Lac operon. However, unlike like the promoter mutations, these mutations don’t respond to the inducer IPTG. These mutations, designated Lac3– and Lac4–, are evaluated for the quantity of ß-galactosidase and permease activity expressed with or without IPTG:

 

Mapping experiments reveal that Lac3– and Lac4– are different short deletions located in the region before the start of the LacZ gene. Given the data shown above suggest which genetic element(s) in addition to part of the promoter has been deleted in each mutant. 

TLS Online TPP Program

#Question id: 15638

#SCPH28 | Zoology

You have isolated two mutations that show decreased expression of the Lac operon. However, unlike like the promoter mutations, these mutations don’t respond to the inducer IPTG. These mutations, designated Lac3– and Lac4–, are evaluated for the quantity of ß-galactosidase and permease activity expressed with or without IPTG:

 

Mapping experiments reveal that Lac3– and Lac4– are different short deletions located in the region before the start of the LacZ gene. Given the data shown above suggest which genetic element(s) in addition to part of the promoter has been deleted in each mutant.