Hydroxyl groups (−OH),  and methoxy groups(−OCH3) modification provides colour variations in case of

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#Question id: 2927

#SCPH01 Biochemistry

Site-directed mutagenesis of Tyr-15 to Phe in cdc2:

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#Question id: 8683

#SCPH28 | Zoology

Use the following information to answer the question.

Refer to the figure. Which of the following forms a monophyletic group?

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#Question id: 13088

#SCPH06 I Botany

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

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#Question id: 10164

#I Life Science/ Life Sciences Group – I-V

C4 photosynthetic carbon cycle is also known as the

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#Question id: 4343

#SCPH01 Biochemistry

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If a researcher moves the repressor gene (lacI), along with its promoter, to a position at some several thousand base pairs away from its normal position, which of the following results would be expected?