How to Phosphofructokinase-1 (PFK-1) and Fructose 1,6-Bisphosphatase are reciprocally regulated ?

a) ATP inhibits PFK-1 by binding to an allosteric site and lowering the affinity of the enzyme for its substrate fructose 6-phosphate

b) citrate is also an allosteric regulator of PFK-1, high citrate concentration increases the inhibitory effect of ATP

c) FBPase-1, is strongly inhibited (allosterically) by AMP; when the cell’s supply of ATP is low (corresponding to high AMP), the ATP-requiring synthesis of glucose slows

d) AMP inhibits PFK-1 by binding to an allosteric site and lowering the affinity of the enzyme for its substrate fructose 6-phosphate

Which of the following statements of regulation of enzyme is correct?

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#Question id: 23151

#SCPH06 I Botany

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?

1. Fluorescence will decrease

2.  Fluorescence will increase

3. Emission of light at higher wavelength

4. Emission of light at lower wavelength

Which of the following options are correct?

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#Question id: 23152

#SCPH06 I Botany

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 

1. Alkaline cells

2. Acidic cells 

3. Dead cells

4. Live cells

Which of the following options are incorrect?

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#Question id: 23153

#SCPH06 I Botany

SNARF-1is a fluorescent dye used to measure which ion concentration?

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#Question id: 23154

#SCPH06 I Botany

When the organelles pH is acidic the how the SNARF-1 dye measures this acidity?

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#Question id: 23155

#SCPH06 I Botany

Match the following ions in column I with their relevant dyes column II.

Column I                                                       Column II

1. K+                                                                 a. Fura-2

2. Ca2+                                                             b. SNARF-1

3. H+                                                                 c. Ion potassium green

Which of the following options is correct?

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#Question id: 23156

#SCPH06 I Botany

Most widely used method of detecting specific proteins, uses an antibody to which a fluorescent dye has been covalently attached