Paclobutrazol and other inhibitors of P450 monooxygenases specifically inhibit the stage of gibberellin biosynthesis

TLS Online TPP Program

#Question id: 10522

#SCPH05 I Biotechnology

Mechanical barriers provide a first line of defence against insect pests and pathogens, these mechanical barriers developed by plants;

COLUMN A	COLUMN B
A. Thorns	i) It can be found on the stem and petioles of roses and are formed by the epidermis
B. Spines	ii) Found on a lemon tree (Citrus sp.) are modified branches, as can be seen by their position in the axil of a leaf
C. Prickles	iii) Found on shoot and leaves of tomato (Solanum lycopersicum) are derived from epidermal cells
D. Trichomes	iv) which are characteristic of cacti (Opuntia spp.) in the New World, are modified leaves

Match correctly following combination of barrier developed by plants ;

TLS Online TPP Program

#Question id: 1655

#I Life Science/ Life Sciences Group – I-V

IgA in seromucus secretions:

TLS Online TPP Program

#Question id: 930

#I Life Science/ Life Sciences Group – I-V

Ketone bodies are formed in the liver and transported to the extrahepatic tissues mainly as:

TLS Online TPP Program

#Question id: 27879

#Research Methodology

Scale in which the respondent directly compares two or more objects and makes choices among them is

TLS Online TPP Program

#Question id: 13088

#SCPH06 I Botany

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;