PAM 250 matrix is normally used for –

TLS Online TPP Program

#Question id: 14359

#SCPH05 I Biotechnology

How many enzyme units are there in 1 katal? 

TLS Online TPP Program

#Question id: 14360

#SCPH05 I Biotechnology

1 pg of an enzyme (Mr 160 000) converts 0.192 pmol/min. This enzyme is made up of four subunits. Calculate turnover number 

TLS Online TPP Program

#Question id: 14361

#SCPH05 I Biotechnology

1 m of an enzyme (Mr 160 000) converts 0.192 i.imol/min. This enzyme is made up of four  subunits. Calculate catalytic centre activity

TLS Online TPP Program

#Question id: 14362

#SCPH05 I Biotechnology

An experiment requires the use of 5 x 107 units of enzyme. The price for the enzyme is quoted for katal amounts. Allowing for a 20% excess for wastage, pipetting, and so forth, what approximate round number of katals of enzyme would you purchase? 

TLS Online TPP Program

#Question id: 14363

#SCPH05 I Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate the specific activities in units/g at each of the two steps 

TLS Online TPP Program

#Question id: 14364

#SCPH05 I Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate % increase in activity obtained over the two step