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#Question id: 15653
#SCPH01 Biochemistry
You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. You have isolated a mutation that causes constitutive melibiase activity, which you designate MelA–. P1 phage mapping experiments using a Tn5 insertion linked to Mel1 show that MelA– is not linked to Mel1. Moreover you find that when an amber suppressor is introduced into a MelA– mutant, normal melibiase regulation is restored. Classify the MelA– mutation on the basis of genetic properties, make a proposal for the type of regulatory functions affected by the MelA– mutation?
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#Question id: 592
#I Life Science/ Life Sciences Group – I-V
The expression: Vmax = k2[E]total applies to
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#Question id: 23248
#SCPH28 | Zoology
Pyrosequencing is sequencing by synthesis whereby a PCR template is hybridised to an oligonucleotide and incubated with
1. DNA polymerase
2. ATP sulphurylase
3. Luciferase
4. Apyrase
Which of the following option has correct combination?
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#Question id: 4901
#SCPH12 I Genetics
Assume that the alleles A (dominant) and a (recessive) show a maternal effect mode of inheritance. In a cross between an Aa female and an aa male, what would be the phenotypes of the progeny?
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#Question id: 1751
#SCPH01 Biochemistry
Which of the following is characteristically produced by the Th2 CD4 cells which provide help for antibody production, but not by Th1 cells?