TLS Online TPP Program

#Question id: 444


Which of the following is not a factor contributing to the large free energy of hydrolysis of ATP?

#SCPH05 I Biotechnology
  1. Electrostatic repulsion of oxygen atoms

  2. Better solvation of products than ATP itself

  3. Complexes with Mg2+ or Mn2+ ions

  4. Better stability of products than ATP itself

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TLS Online TPP Program

#Question id: 12916

#SCPH06 I Botany

Which statement is correct for Zoo blotting?

TLS Online TPP Program

#Question id: 12916

#SCPH28 | Zoology

Which statement is correct for Zoo blotting?

TLS Online TPP Program

#Question id: 12917

#SCPH01 Biochemistry

Arrange the steps of stages in PCR in correct order.
1. The region to be specifically amplified is made accessible within the complex DNA.
2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.
3. A thermal cycler is used to incrementally decrease the annealing temperature.
4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

TLS Online TPP Program

#Question id: 12917

#SCPH06 I Botany

Arrange the steps of stages in PCR in correct order.
1. The region to be specifically amplified is made accessible within the complex DNA.
2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.
3. A thermal cycler is used to incrementally decrease the annealing temperature.
4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

TLS Online TPP Program

#Question id: 12917

#SCPH28 | Zoology

Arrange the steps of stages in PCR in correct order.
1. The region to be specifically amplified is made accessible within the complex DNA.
2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.
3. A thermal cycler is used to incrementally decrease the annealing temperature.
4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

TLS Online TPP Program

#Question id: 12918

#SCPH01 Biochemistry

Name the enzyme which helps in the DNA synthesis in the PCR.