Calculate the equilibrium constant for an oxidation-reduction reaction involving the transfer of 1 mole of electrons with DE0ʹ= 0.25 V. (R = 8.315 JK-1mol-1, F = 96.48 kJV-1mol-1, T = 298 K)

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#Question id: 23170

#SCPH06 I Botany

Which epitope tags can be used to tag recombinant proteins?

1. FLAG

2. myc

3. TICK

Which of the following options is incorrect?

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#Question id: 23171

#SCPH06 I Botany

Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize

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#Question id: 23172

#SCPH06 I Botany

Green fluorescent protein do contains a tripeptide sequence i.e. ser-tyr-gly but it only fluoresce when 

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#Question id: 23173

#SCPH06 I Botany

There are various drawbacks still in fluorescence microscopy such as

1.  Light emitted comes from molecules above and below the plane of focus

2. Blurred image due to superposition of fluorescent images

Which of the above statement is/ are correct?

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#Question id: 23175

#SCPH06 I Botany

If we use a high-intensity light on to the fluorochrome there will initially be 

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#Question id: 23176

#SCPH06 I Botany

When we use a high-intensity light to permanently bleach the fluorochrome in the patch, there will initially be no fluorescence and it will look