TLS Online TPP Program

#Question id: 554


The Lineweaver-Burk plot is used to:

#SCPH05 I Biotechnology
  1. determine the equilibrium constant for an enzymatic reaction.

  2. extrapolate for the value of reaction rate at infinite enzyme concentration.

  3. illustrate the effect of temperature on an enzymatic reaction.

  4. solve, graphically, for the rate of an enzymatic reaction at infinite substrate concentration.

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TLS Online TPP Program

#Question id: 16074

#SCPH01 Biochemistry

Match the items in Group 1 with an appropriate description in Group 2 
Group 1                                                    Group 2 
P. PSI-BLAST                                    1. ungapped, aligned motif 
Q. CLUSTALW                                 2. Profiles
R. BLOCK                                         3. Heuristic method
S. FASTA                                           4. Multiple sequence alignment

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#Question id: 5752

#SCPH28 | Zoology

base change that changes a codon for an amino acid to a stop codon

TLS Online TPP Program

#Question id: 5578

#SCPH28 | Zoology

The phase change of an apical meristem from the juvenile to the mature vegetative phase is often revealed by ________.

TLS Online TPP Program

#Question id: 13058

#SCPH28 | Zoology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following true about real time PCR?

TLS Online TPP Program

#Question id: 11089

#I Life Science/ Life Sciences Group – I-V

A 45-year-old man inhaled as much air as possible and then expired with a maximum effort until no more air could be expired. This produced the maximum expiratory flow-volume curve shown in the following diagram. What is the forced vital capacity of this man (in liters)?