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#Question id: 13087
#SCPH28 | Zoology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?
TLS Online TPP Program
#Question id: 15869
#SCPH05 I Biotechnology
In a modest laboratory, aseptic handling, maintenance and observation of cultures, these activities can be done in the culture room. Some facilities are required for the culture room, except?
TLS Online TPP Program
#Question id: 15148
#SCPH12 I Genetics
You have isolated a set of five yeast mutants that form dark red colonies instead of the usual white colonies of wild-type yeast. You cross each of the mutants to a wild-type haploid strain and obtain the results shown below;
you cross each haploid mutant strain to a different haploid mutant of the opposite mating type. What type of mutation shown in the above figure;
TLS Online TPP Program
#Question id: 3517
#SCPH06 I Botany
The following two genotypes are crossed: AaBbCcddX AabbCcDd. What will number of different genotypes among the progeny of this cross?
TLS Online TPP Program
#Question id: 1784
#SCPH05 I Biotechnology
which parts are similar among the three pathways