TLS Online TPP Program

#Question id: 10392


Structural domains in phytochrome are dimeric chromoproteins. The two sub‐units of phytochromes are linked with

#SCPH05 I Biotechnology
  1. GAF domain
  2. PRD domain
  3. Kinase domain
  4. Hinge region  
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TLS Online TPP Program

#Question id: 13057

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
How primer concentration leads to decrease in PCR efficiency?

TLS Online TPP Program

#Question id: 10284

#I Life Science/ Life Sciences Group – I-V

Allosteric regulation of enzyme multisubunit or multiactive site shows cooperativity with the sigmoidal graph between substerate conc. v/s Activity,


a) Cell contain NADH, FADH2 and ATP, that inhibits the allosteric enzyme activity by lowering the affinity with its substate, graph shifts towards B from control

 b) A- shows low ATP, the Km for fructose 6-phosphate is relatively low, the affinity of the enzyme with its substate is relatively high

 c) Haemoglobin, Phosphofructokinase, Phosphate dehydrogenase complex, α-ketoglutarate dehydrogenase complex, these all enzymes shows characteristics of sigmoidal graph

 d) When ATP is high, Km for fructose 6-phosphate is greatly increased- this activity shown by B in the graph

 e) fructose 2,6-bisphosphate binds to its allosteric site on PFK-1, it increases the enzyme’s affinity for its substrate fructose 6-phosphate and reduces its affinity for the allosteric inhibitors ATP and citrate- shown in graph by A

 Which of the following  combination of the enzyme activity with its substate correctly shown;

TLS Online TPP Program

#Question id: 2634

#SCPH05 I Biotechnology

Tryptophan, the end-product of the enzymes encoded by the trp operon, therefore acts as a co-repressor and inhibits its own synthesis through:

TLS Online TPP Program

#Question id: 4600

#SCPH28 | Zoology

Scientific theories ________.

TLS Online TPP Program

#Question id: 8694

#SCPH28 | Zoology

Which of the following is (are) problematic when the goal is to construct phylogenies that accurately reflect evolutionary history?