Integral membrane proteins that form water-selective channels across the membrane, Because water diffuses faster through such channels than through a lipid bilayer

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#Question id: 13093

#SCPH28 | Zoology

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                    

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.

 

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#Question id: 13070

#SCPH05 I Biotechnology

Antibodies raised against an antigen from one organism will react to a greater or lesser degree with similar antigens from a related organism, depending on how many surface epitopes they share. This has given rise to a systematic method of identification of microorganisms known as______?

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#Question id: 1671

#SCPH05 I Biotechnology

A chromosome on which T-cell receptor α chain gene rearrangement has occurred lacks which of the following gene segments?

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#Question id: 3044

#I Life Science/ Life Sciences Group – I-V

If the pH indicator were left out of MacConkey agar, the medium would be

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#Question id: 1569

#I Life Science/ Life Sciences Group – I-V

A bacterium entering the body through a small cut in the skin will do which of the following?