Dot matrix analysis of the amino acid sequences of lambda phage cI (horizontal sequence) and phage P22 c2 (vertical sequence) repressors is shown below. Which one of the following is correct?

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#Question id: 23166

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To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?

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#Question id: 23167

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A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.

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#Question id: 23168

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What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?

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#Question id: 23169

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Indirect immunofluorescence microscopy uses an unlabeled primary antibody, followed by a fluorescently labeled 

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#Question id: 23170

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Which epitope tags can be used to tag recombinant proteins?

1. FLAG

2. myc

3. TICK

Which of the following options is incorrect?

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#Question id: 23171

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Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize