In strong acid and at elevated temperatures, for example perchloric acid (HClO4) at more than 100°C, nucleic acids tend to:

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#Question id: 5230

#SCPH06 I Botany

Homeotic genes

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#Question id: 13090

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 3’s plasmid is;

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#Question id: 4807

#SCPH06 I Botany

Three incompletely link marker gene was found. There is a marker A gene present in middle. Cross between aaBBcc X AAbbCC to produce heterozygous were allowed test cross, which of the following test cross offspring should be least frequent?

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#Question id: 5739

#SCPH06 I Botany

Polyploid plants found in nature usually have even numbers of chromosomes because organisms having odd numbers

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#Question id: 10688

#SCPH06 I Botany

For two species A and B in competition, the carrying capacities, competition coefficients, per capita growth rate and population size are

KA = 1000           KB = 1200    α of B= 1     β of A= .5       rB= .5         r A= .3                

AN= 500                  BN= 200

According to the Lotka-Volterra model, what is growth rate of species A?