Which of the following experimental requirements makes an siRNA approach unsuitable for determining the consequence of silencing a gene of interest in an established cell line?

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#Question id: 12744

#SCPH06 I Botany

In Agglutination process, the precipitin bands will be more or less pronounced it depends on the factor___

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#Question id: 5235

#SCPH06 I Botany

What is proteomics?

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#Question id: 14197

#SCPH05 I Biotechnology

What is the generation time of a bacterial population that increases from  10,000 cells to 10,000,000 cells in four hours of growth?

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#Question id: 13092

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 821

#I Life Science/ Life Sciences Group – I-V

Why the thermophilic bacteria’s are not melted at the temp on which the most of the proteins have melts?