With respect to microRNAs, which, if any, of the following statements, is FALSE?)

A. MicroRNA genes often occur in gene clusters within introns of protein-coding genes and are usually transcribed by RNA polymerase II.

B. Like an mRNA a miRNA is initially produced as a larger precursor RNA that, like the great majority of mRNAs, usually has a 5’ cap and a poly(A) tail.

C. MicroRNAs are produced by germ-line cells only.

D. A miRNA is initially produced as a duplex RNA but in order to work it needs to be converted into a single-strand RNA.

E. MicroRNAs usually work as transcription factors.

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#Question id: 23166

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To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?

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#Question id: 23167

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A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.

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#Question id: 23168

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What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?

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#Question id: 23169

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Indirect immunofluorescence microscopy uses an unlabeled primary antibody, followed by a fluorescently labeled 

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#Question id: 23170

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Which epitope tags can be used to tag recombinant proteins?

1. FLAG

2. myc

3. TICK

Which of the following options is incorrect?

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#Question id: 23171

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Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize