miRNAs are encoded in the genome as segments of longer transcripts. Their characteristic structure helps identify them and predict the target genes they might regulate. Which of the following are the characteristic features of these small RNA molecules?

I. The functional form of an miRNA is typically 21 or 22 nucleotides (it can vary from 19 to 25 nucleotides).

II. The first cleavage liberates the stem-loop, called the pre-miRNA; the second generates the mature miRNA from the pre-miRNA.

III. The pre-miRNAs can be encoded by any part of a transcript: that is, they might fall within coding regions, within leader regions, or within Introns.

IV. The base pairing between miRNA and target RNA is initiated by interactions of so-called seed residues—the sequence between bases 2 and 9 of the 22-nucleotide miRNA.

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#Question id: 23248

#SCPH06 I Botany

Pyrosequencing is sequencing by synthesis whereby a PCR template is hybridised to an oligonucleotide and incubated with 

1. DNA polymerase

2.  ATP sulphurylase

3. Luciferase 

4. Apyrase

Which of the following option has correct combination?

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#Question id: 119

#SCPH05 I Biotechnology

Tay-Sachs disease is the result of a genetic defect in the metabolism of:

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#Question id: 1032

#SCPH06 I Botany

A research laboratory is investigating environmental factors that would inhibit the growth of Archaea. One question they have is if adding the antibiotic penicillin would be effective in controlling their growth. What do you think the outcome would be if they tried this?

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#Question id: 346

#SCPH05 I Biotechnology

The partial negative charge in a molecule of water occurs because ________.

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#Question id: 5251

#SCPH28 | Zoology

What do transduction, transformation, and conjugation have in common?