Which of the following is not known to be involved in initiation by eukaryotic RNA polymerase II?

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#Question id: 14663

#SCPH05 I Biotechnology

Identify the file format given below:

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#Question id: 13092

#SCPH06 I Botany

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 2947

#SCPH28 | Zoology

You perform an experiment where you prevent Cdc20 from binding to APC  in a cell line that usually completes the cell cycle in 60 minutes. Your experiment prevents Cdc20 from binding to APC for 120 minutes, and then you fix and stain the DNA, securin, separase and cohesin proteins. Which of the following would NOT be found in the observed cell?

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#Question id: 437

#SCPH05 I Biotechnology

Which thermodynamic quantity is used to determine if a reaction in a cell is spontaneous?

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#Question id: 1545

#SCPH05 I Biotechnology

B cells negatively regulated by,