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TLS Online TPP Program
#Question id: 13055
#SCPH06 I Botany
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following will provide least specific amplification in qPCR?
TLS Online TPP Program
#Question id: 14283
#SCPH05 I Biotechnology
Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3?
TLS Online TPP Program
#Question id: 11566
#SCPH28 | Zoology
If a person loses a large amount of water in a short period of time, he or she may die from dehydration. Antidiuretic hormone (ADH) can help reduce water loss through its interaction with its target cells in the ________.
TLS Online TPP Program
#Question id: 2271
#I Life Science/ Life Sciences Group – I-V
Depolymerization of microtubules is inhibited by
TLS Online TPP Program
#Question id: 18377
#SCPH28 | Zoology
Why xenobiotic compounds are considered ad recalciterants?