Practice Questions

  1. Home
  2. Free Resources
  3. Practice Questions
  4. 41360
TLS Online TPP Program

#Question id: 4167

Which statement is false concerning ribosomes and their components?

#SCPH05 I Biotechnology

  1. All ribosomes have two subunits of unequal size.

  2. The genes for eukaryotic rRNA occur as tandem arrays of hundreds of copies.

  3. The 30S and 50S ribosomal subunits of E. coli combine to generate an 80S subunit.

  4. Sequences exist in prokaryotic and eukaryotic rRNA that are so similar, they likely derive from a common ancestor.

More Questions
TLS Online TPP Program

#Question id: 14251

#SCPH05 I Biotechnology

Yeast cells are recovered from a fermentation broth by using a tubular centrifuge. Sixty percent (60%) of the cells are recovered at a flow rate of 12 l/min with a rotational speed of 4000 rpm. Recovery is inversely proportional to flow rate. a. To increase the recovery of cells to 95% at the same flow rate, what should be the rpm of the centrifuge?
____________________
TLS Online TPP Program

#Question id: 14252

#SCPH05 I Biotechnology

Yeast cells are recovered from a fermentation broth by using a tubular centrifuge. Sixty percent (60%) of the cells are recovered at a flow rate of 12 l/min with a rotational speed of 4000 rpm. Recovery is inversely proportional to flow rate. At a constant rpm of 4000 rpm, what should be the flow rate to result in 95% cell recovery?
________________-
TLS Online TPP Program

#Question id: 14253

#SCPH05 I Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the volume of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 
TLS Online TPP Program

#Question id: 14254

#SCPH05 I Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the height of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 
TLS Online TPP Program

#Question id: 14255

#SCPH05 I Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine the effluent Mab concentration of the system?
TLS Online TPP Program

#Question id: 14256

#SCPH05 I Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine productivity of the system? __________________