What would occur if the repressor of an inducible operon were mutated so it could not bind the operator?

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#Question id: 13053

#SCPH01 Biochemistry

What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?

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#Question id: 1733

#SCPH01 Biochemistry

The early increase in phospholipase Cγ1 activity following T-cell stimulation:

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#Question id: 8855

#SCPH06 I Botany

A spongeʹs structural materials (spicules, spongin) are manufactured by the

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#Question id: 13087

#SCPH28 | Zoology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 4799

#SCPH06 I Botany

You set up a mapping cross involving your favorite gene (YFG) in Neurospora and determine the following octad types: 4:4 arrangement 83; 2:4:2 arrangement 7; 2:2:2:2 arrangement 10. What is the distance between your gene and the centromere?