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TLS Online TPP Program
#Question id: 13062
#SCPH06 I Botany
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?
TLS Online TPP Program
#Question id: 764
#SCPH05 I Biotechnology
Which of the following is NOT correct about tRNA?
TLS Online TPP Program
#Question id: 5062
#SCPH06 I Botany
Which of the signaling pathway is represent in this figure?
TLS Online TPP Program
#Question id: 15489
#SCPH01 Biochemistry
Glyphosate resistance, Aromatic amino acid are all produced by targeting which pathway
TLS Online TPP Program
#Question id: 427
#SCPH28 | Zoology
The degradation of which class of biochemicals does not significantly contribute to the release of energy to cells?