TLS Online TPP Program

#Question id: 4341


Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If a researcher moves the promoter for the lac operon to the region between the beta galactosidase (lacZ) gene and the permease (lacY) gene, which of the following results would be most likely?

#SCPH05 I Biotechnology
  1. The three genes of the lac operon will be expressed normally.

  2. RNA polymerase will no longer transcribe permease.

  3. The operon will still transcribe the lacZ and lacY genes, but the mRNA will not be translated.

  4. Beta galactosidase will not be produced.

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#Question id: 17655

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In DNA isolation, for protein free solution what will be the ratio at  nm

TLS Online TPP Program

#Question id: 17655

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In DNA isolation, for protein free solution what will be the ratio at  nm

TLS Online TPP Program

#Question id: 17655

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In DNA isolation, for protein free solution what will be the ratio at  nm

TLS Online TPP Program

#Question id: 17659

#SCPH01 Biochemistry

During your study of the gene for an enzyme you have isolated an amber mutation in the middle of the gene which produces a truncated form of the enzyme. By placing a +1 frameshift mutation a short distance before the amber mutation and a –1 frameshift mutation a short distance after the amber mutation you create a triple mutant that restores expression of a functional, full-length enzyme. 
a) +1 frameshift puts the amber mutation out of frame so that it is no longer read as a stop.
b) -1 frameshift is inserted after the amber so that no longer read as a stop.
c)  -1 frameshift is inserted after the amber to restore the frame of the end of the protein.
d) +1 frameshift puts the amber mutation out of frame so that it restore the frame of the end of the protein.
Which of the following is the correct prediction about the frame shift mutation?