Which one of the following combinations of secondary metabolite biosynthetic pathway found in the biosynthesis of terpenes?

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#Question id: 15736

#SCPH01 Biochemistry

In shoot regeneration which attribute may have an inhibitory effect on it

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#Question id: 1423

#SCPH28 | Zoology

Which of the following is a characteristic of malignant tumors?

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#Question id: 4309

#SCPH05 I Biotechnology

Imagine that you've isolated a yeast mutant that contains histones resistant to acetylation. What phenotype would you predict for this mutant?

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#Question id: 13088

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

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#Question id: 4738

#SCPH06 I Botany

Red kernel color in wheat results from the presence of at least one dominant allele of each of two independently segregating genes . Kernels on rr bb plants are white, and the genotypes R— bb and rr B— result in brown kernel color. Suppose that plants of a variety that is true breeding for red kernels are crossed with plants true breeding for white kernels produced F1 were allowed to selfing .what are F2 phenotype ratio ?