Endospores are

TLS Online TPP Program

#Question id: 342

#I Life Science/ Life Sciences Group – I-V

In a single molecule of water, two hydrogen atoms are bonded to a single oxygen atom by ________.

TLS Online TPP Program

#Question id: 758

#I Life Science/ Life Sciences Group – I-V

Both miRNAs and one strand of siRNAs are bound by proteins to form a ribonucleoprotein complex called:

TLS Online TPP Program

#Question id: 4951

#SCPH28 | Zoology

The synthesis of new DNA requires the prior existence of oligonucleotides to serve as primers. On Earth, these primers are small RNA molecules. This latter observation is evidence in support of the hypothesized existence of

TLS Online TPP Program

#Question id: 13090

#SCPH05 I Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 3’s plasmid is;

TLS Online TPP Program

#Question id: 19453

#SCPH01 Biochemistry

What will be the value of resolution when the angle given is 60O  and R.I. given is 1.3 at 340nm?