TLS Online TPP Program

#Question id: 5265


The mechanism by which genes are transferred into bacteria via viruses is called

#SCPH05 I Biotechnology
  1. replica plating.

  2. transformation.

  3. transduction.

  4. conjugation.

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TLS Online TPP Program

#Question id: 13032

#I Life Science/ Life Sciences Group – I-V

In SDS-PAGE, protein sample is first treated with detergent sodium dodecyl sulfate (SDS), in order to

TLS Online TPP Program

#Question id: 2122

#SCPH28 | Zoology

The inner (plasma) membrane of E. coli is about 75% lipid and 25% protein by weight.  How many molecules of membrane lipid are there for each molecule of protein?  (Assume that the average protein is Mr 50,000 and the average lipid is 750.)

TLS Online TPP Program

#Question id: 13076

#SCPH28 | Zoology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

TLS Online TPP Program

#Question id: 1680

#SCPH05 I Biotechnology

Where are the MHC class II region genes in the mouse located?

TLS Online TPP Program

#Question id: 20847

#SCPH06 I Botany

In groundwater hydrocarbon spill which metal reduction was the major electron accepting process?