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#Question id: 13087
#SCPH28 | Zoology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?
TLS Online TPP Program
#Question id: 3512
#SCPH01 Biochemistry
How many different type of gamete produced by plant having genotype AABBccDd?
TLS Online TPP Program
#Question id: 14804
#SCPH05 I Biotechnology
Calculate the percentage sequence identity for the pairwise alignment given below:
H E L L O _
Y E L L O W
TLS Online TPP Program
#Question id: 8891
#SCPH06 I Botany
While vacationing in a country that lacks adequate meat inspection, a student ate undercooked ground beef. Sometime later the student became easily fatigued, and lost body weight. At about the same time, whitish, flattened, rectangular objects full of small white spheres started appearing in his feces. Administration of niclosamide cured the problem. The student had probably been infected by a
TLS Online TPP Program
#Question id: 22937
#I Life Science/ Life Sciences Group – I-V
Gene for regulatory pathway that promotes flowering of Arabidopsis in long days: