TLS Online TPP Program

#Question id: 383


Three buffers are made by combining a 1 M solution of acetic acid with a 1 M solution of sodium acetate in the ratios shown below

1 M acetic acid              

1 M sodium acetate  

Buffer 1: 10 mL

 90 mL

Buffer 2: 50 mL

50 mL

Buffer 3: 90 mL

10 mL

 Which of these statements is true of the resulting buffers?

#SCPH05 I Biotechnology
  1. pH of buffer 1 < pH of buffer 2 < pH of buffer 3

  2. pH of buffer 1 = pH of buffer 2 = pH of buffer 3

  3. pH of buffer 1 > pH of buffer 2 > pH of buffer 3

  4. The problem cannot be solved without knowing the value of pKa.

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TLS Online TPP Program

#Question id: 13062

#SCPH06 I Botany

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

TLS Online TPP Program

#Question id: 3645

#SCPH28 | Zoology

The single-stranded bubble created in DNA replication is coated with _____________ to protect it from breakage and to prevent the DNA renaturing.

TLS Online TPP Program

#Question id: 5765

#SCPH28 | Zoology

How many different trisomics could be formed in a plant with a diploid number of 12?

TLS Online TPP Program

#Question id: 15604

#SCPH05 I Biotechnology

The bacteria which cause septicaemia are fast becoming resistant to the antibiotics used for controlling them. Which one of the following explanations fits best?

TLS Online TPP Program

#Question id: 11755

#SCPH28 | Zoology

Which of the following solutions when infused intravenously would result in an increase in extracellular fluid volume, a decrease in intracellular fluid volume, and an increase in total body water after osmotic equilibrium?