Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the height of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

TLS Online TPP Program

#Question id: 15727

#SCPH01 Biochemistry

The stationary phase is forced on the culture by , identify correct option

1. Depletion of the nutrients

2. Due to accumulation of cellular wastes

3. Accumulation of the nutrients

4. Removal of cellular wastes

TLS Online TPP Program

#Question id: 15727

#SCPH05 I Biotechnology

The stationary phase is forced on the culture by , identify correct option

1. Depletion of the nutrients

2. Due to accumulation of cellular wastes

3. Accumulation of the nutrients

4. Removal of cellular wastes

TLS Online TPP Program

#Question id: 15727

#SCPH28 | Zoology

The stationary phase is forced on the culture by , identify correct option

1. Depletion of the nutrients

2. Due to accumulation of cellular wastes

3. Accumulation of the nutrients

4. Removal of cellular wastes

TLS Online TPP Program

#Question id: 15728

#SCPH01 Biochemistry

Single cells can be cultured using the following techniques:

(1) Filter paper raft-nurse tissue technique

(2) Microchamber technique

(3) Microdrop method

(4) Bergman's plating technique

(5) Thin layer liquid medium

Select the option with correct choices.

TLS Online TPP Program

#Question id: 15728

#SCPH05 I Biotechnology

Single cells can be cultured using the following techniques:

(1) Filter paper raft-nurse tissue technique

(2) Microchamber technique

(3) Microdrop method

(4) Bergman's plating technique

(5) Thin layer liquid medium

Select the option with correct choices.

TLS Online TPP Program

#Question id: 15728

#SCPH28 | Zoology

Single cells can be cultured using the following techniques:

(1) Filter paper raft-nurse tissue technique

(2) Microchamber technique

(3) Microdrop method

(4) Bergman's plating technique

(5) Thin layer liquid medium

Select the option with correct choices.