Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine the effluent Mab concentration of the system?

TLS Online TPP Program

#Question id: 13056

#SCPH28 | Zoology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

TLS Online TPP Program

#Question id: 10889

#SCPH06 I Botany

Which of the following ecosystem that is lower Productivity/Biomass ratios and energy transfer rates to higher trophic levels are slower 

TLS Online TPP Program

#Question id: 292

#I Life Science/ Life Sciences Group – I-V

Which of the following types of non-covalent bonds or interactions is responsible for forming the α helix and β sheet secondary structural motifs of a protein:

TLS Online TPP Program

#Question id: 2730

#SCPH05 I Biotechnology

The histone tails are required for chromatin to condense from the beads-on- a-string conformation into the 30-nm fiber. Recent experiments indicate that the N-terminal tails of histone H4, particularly_____________, are critical for forming the 30-nm fiber.

TLS Online TPP Program

#Question id: 9189

#SCPH28 | Zoology

A plant will recognize a pathogenic invader