Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine productivity of the system? __________________

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#Question id: 14349

#SCPH05 I Biotechnology

An aerated bioreactor will increase oxygen transfer rates with the addition of detergents because it 

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#Question id: 5342

#SCPH06 I Botany

Women having normal color vision, normal pigmentation and A blood group whose father is color blindness, albinism and O blood group will marry with men having colorblind, albinism and O blood group. What is probability of their first child will colorblindness son, normal pigmentation and O blood group? 

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#Question id: 2075

#SCPH01 Biochemistry

The force driving simple diffusion is:

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#Question id: 13064

#SCPH06 I Botany

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 3611

#SCPH01 Biochemistry

ABO blood group system is due to