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TLS Online TPP Program
#Question id: 13087
#SCPH05 I Biotechnology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?
TLS Online TPP Program
#Question id: 18590
#SCPH28 | Zoology
Origin of life starts from which geological time period?
TLS Online TPP Program
#Question id: 4344
#SCPH28 | Zoology
How does the transcription of structural genes in an inducible operon occur?
TLS Online TPP Program
#Question id: 2740
#SCPH05 I Biotechnology
Escherichia coli use antisense regulation to switch the expression of outer membrane proteins. Antisense _____________.
TLS Online TPP Program
#Question id: 11912
#SCPH06 I Botany
The success of birds introduced to Tahiti or the Hawaiian Islands was dependent on the morphology of the species already present. If there was a small morphological difference in bill length between the resident and introduced species, then