Practice Questions

TLS Online TPP Program

#Question id: 13102

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.)
You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.

Why is it that having more repeats leads to a band that is higher in the gel?

#SCPH05 I Biotechnology

Because they are homozygotes at this region
The more repeats you have, the longer your DNA fragment is, and longer DNA fragments migrate more slowly in gels.
Because they are heterozygotes at this region
The less no. of repeats you have, the longer your DNA fragment is, and longer DNA fragments migrate more slowly in gels.