To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 1552

#SCPH05 I Biotechnology

A common clinical pathological finding during a viral infection is

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#Question id: 5701

#SCPH28 | Zoology

Cross-over suppressors are production of gametes having cross over products is lethal. Which of the following statement is incorrect?

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#Question id: 19611

#SCPH05 I Biotechnology

During change of O2 to O₂^2- ion, the electron adds on which of the following orbitals?

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#Question id: 9162

#SCPH06 I Botany

If the direction of Earth's rotation reversed, the most predictable effect would be ________.

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#Question id: 7296

#I Life Science/ Life Sciences Group – I-V

some of the following statements regarding to this experiment are given below. Which of the following statements is incorrect?