To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 19644

#SCPH05 I Biotechnology

When acetylene is treated with HBr, the product is ———-

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#Question id: 9225

#SCPH06 I Botany

Use the following figure to answer the question

In looking at the species-area plot in the figure, what can be concluded?

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#Question id: 413

#SCPH28 | Zoology

The hydrolysis of ATP has a large negative DG'°; nevertheless it is stable in solution due to:

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#Question id: 8918

#SCPH28 | Zoology

In individual insects of some species, whole chromosomes that carry larval genes are eliminated from the genomes of somatic cells at the time of metamorphosis. A consequence of this occurrence is that

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#Question id: 7296

#SCPH06 I Botany

some of the following statements regarding to this experiment are given below. Which of the following statements is incorrect?