Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

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#Question id: 5043

#I Life Science/ Life Sciences Group – I-V

 The ability of a single cell to divide and produce all of the differentiated cells in an organism:

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#Question id: 5043

#SCPH06 I Botany

 The ability of a single cell to divide and produce all of the differentiated cells in an organism:

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#Question id: 5043

#SCPH28 | Zoology

 The ability of a single cell to divide and produce all of the differentiated cells in an organism:

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#Question id: 5044

#SCPH01 Biochemistry

The cell might not look different from its nearest or most distant neighbors in the embryo and show no visible signs of differentiation, but its developmental fate has become restricted, the process known as-

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#Question id: 5044

#I Life Science/ Life Sciences Group – I-V

The cell might not look different from its nearest or most distant neighbors in the embryo and show no visible signs of differentiation, but its developmental fate has become restricted, the process known as-

TLS Online TPP Program

#Question id: 5044

#SCPH06 I Botany

The cell might not look different from its nearest or most distant neighbors in the embryo and show no visible signs of differentiation, but its developmental fate has become restricted, the process known as-