Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 15210

#SCPH28 | Zoology

Which of the following buffer gives superior separation of smaller fragments (< 2 kb) of miRNA prudentially

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#Question id: 19458

#SCPH01 Biochemistry

How phase contrast and DIC microscopy are used to image live cells and unstained tissues?

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#Question id: 14770

#I Life Science/ Life Sciences Group – I-V

Which of the following is a sequence alignment tool?

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#Question id: 23186

#SCPH06 I Botany

Which of the following are super resolution microscopy.

1. Total internal reflection microscopy

2. Stimulated emission depletion microscopy

3. Spinning disk confocal microscope

4. Photo-activated localization microscopy

Which of the following option has correct combination?

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#Question id: 15603

#SCPH05 I Biotechnology

Novel antibiotic can be made by