Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

#Question id: 4749

#SCPH12 I Genetics

A normal woman, whose father was Hemophilic and color blind is married to a colorblind, nonhemophilic man. What will be probability of colorblind, hemophilic daughter?

#Question id: 4750

#SCPH01 Biochemistry

Which of the following is similarity about Sex-limited Characteristics (SLC) and genomic imprinting (GI)?

#Question id: 4750

#SCPH12 I Genetics

Which of the following is similarity about Sex-limited Characteristics (SLC) and genomic imprinting (GI)?

#Question id: 4751

#SCPH01 Biochemistry

In the following example, 3 independently assorting genes are known to govern coat color in mice. The genotype of few of the coat colors is given below:

Agouti: A-B-C- Black: aa B-C - Albino: -- -- cc

What will be the expected frequency of abino, in progeny from crosses of  AaBbCc with albino of the genotype aabbcc?

#Question id: 4751

#SCPH12 I Genetics

In the following example, 3 independently assorting genes are known to govern coat color in mice. The genotype of few of the coat colors is given below:

Agouti: A-B-C- Black: aa B-C - Albino: -- -- cc

What will be the expected frequency of abino, in progeny from crosses of  AaBbCc with albino of the genotype aabbcc?

#Question id: 4752

#SCPH01 Biochemistry

Red-green color blindness is caused by a sex-linked recessive allele. A color-blind man marries a woman with normal vision whose father was color-blind. What is the probability that they will have a color-blind daughter? What is the probability that their first son will be color-blind respectively?