Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the statement is worng? Aminopetrin………s

TLS Online TPP Program

#Question id: 2309

#SCPH06 I Botany

Membrane-bound compartments in eukaryotic cells are called:

TLS Online TPP Program

#Question id: 11527

#I Life Science/ Life Sciences Group – I-V

Coleoptiles are very sensitive to light, if illuminated on one side with a short pulse of dim blue light, it will

TLS Online TPP Program

#Question id: 5240

#SCPH28 | Zoology

If bacteria grow in the presence of a large amount of DNase, which of the following recombination process completely inhibited?

TLS Online TPP Program

#Question id: 15607

#SCPH01 Biochemistry

In gene transformation over high expression can be achieved by expressing the gene 

1. Inducible promoter

2. Constitutive  promoter

3. Terminator sequence

4. Increasing copy no. of gene

Which of the following are correct

TLS Online TPP Program

#Question id: 501

#SCPH05 I Biotechnology

In a tissue that metabolizes glucose via the pentose phosphate pathway, C-1 of glucose would be expected to end up principally in: