Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

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#Question id: 2258

#SCPH01 Biochemistry

The arrangement of microtubules in eukaryotic flagella is referred to as

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#Question id: 19355

#SCPH28 | Zoology

Genes are often transferred from eukaryotes into prokaryotes with the prime objective

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#Question id: 5223

#SCPH06 I Botany

In order to determine the probable function of a particular sequence of DNA in humans, what might be the most reasonable approach?

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#Question id: 11073

#I Life Science/ Life Sciences Group – I-V

The respiratory passageways have smooth muscle in their walls. Which of the following best describes the effect of acetylcholine and epinephrine on the respiratory passageways?

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#Question id: 12907

#SCPH28 | Zoology

The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____