Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

TLS Online TPP Program

#Question id: 15923

#SCPH05 I Biotechnology

The growth of animal cells in vitro in a suitable culture medium is called___________

TLS Online TPP Program

#Question id: 763

#SCPH05 I Biotechnology

Which of the following could be an effect of miRNA regulation?

TLS Online TPP Program

#Question id: 11274

#SCPH06 I Botany

Assimilation efficiency (AE) is the proportion of ingested energy assimilated in to blood stream but Production efficiency (PE) is the proportion of assimilated energy that is incorporated in to biomass. Following some statement is correct?

A. Assimilation efficiencies of aquatic herbivore is greater than terrestrial herbivore

B. Production efficiencies of higher tropic level are greater than lower tropic level

C. production efficiencies of vertebrate is less than invertebrate

D. Assimilation efficiencies of Carnivore are often greater than herbivore

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#Question id: 18605

#SCPH01 Biochemistry

Thermal modulation are used in PCR for the functions of

TLS Online TPP Program

#Question id: 2641

#SCPH05 I Biotechnology

Which segments of the attenuator together form the repression loop?