Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

#Question id: 9159

#SCPH06 I Botany

Which of the following investigations would shed the most light on the future distribution of organisms in temperate regions that are faced with climate change?

#Question id: 3536

#SCPH01 Biochemistry

What is the probability that selfing an F1 plant from Mendel dihybrid cross ( RrYy X RrYy) will produce an offspring with an rrYY genotype?

#Question id: 8695

#SCPH28 | Zoology

Phylogenetic trees constructed from evidence from molecular systematics are based on similarities in ________.

#Question id: 3483

#SCPH28 | Zoology

Which of the following statements about evolution of behavior is correct?

A) Natural selection will favor behavior that enhances survival and reproduction.

B) An animal may show behavior that maximizes reproductive fitness.

C) If a behavior is less than optimal, it is not completely evolved but will eventually,become optimal.

#Question id: 3006

#SCPH28 | Zoology

A bacterial population increases from 10³ cells to 10⁹ cells in 10 h. Calculate the number of generation time in minute.