Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 747

#I Life Science/ Life Sciences Group – I-V

In nucleic acids, the bases are covalently attached to the 1-position of a pentose sugar ring, to form a:

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#Question id: 10981

#I Life Science/ Life Sciences Group – I-V

Which of the following structures will have the slowest rate of conduction of the cardiac action potential?

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#Question id: 4191

#SCPH28 | Zoology

How many GTP are hydrolyzed for every aminoacyl-tRNA that is successfully inserted into the A site of the ribosome?

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#Question id: 15697

#I Life Science/ Life Sciences Group – I-V

Which of the following does not describe PAM matrices?

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#Question id: 18639

#SCPH01 Biochemistry

Which one of the following techniques is used for the evaluation of phylogenetic trees?