Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

#Question id: 7767

#SCPH01 Biochemistry

How can evolution select for phenotypes that postpone reproduction or sexual maturity?” There is often a trade-off between reproduction and maintenance, and in many species reproduction and senescence are closely linked. Recent studies of mice, Caenorhabditis elegans, and Drosophila suggest that there is a conserved genetic pathway that regulates aging and that it can indeed be selected for. This pathway involves the response to insulin and insulin-like growth factors, Name of these transcription factors are

#Question id: 7767

#I Life Science/ Life Sciences Group – I-V

How can evolution select for phenotypes that postpone reproduction or sexual maturity?” There is often a trade-off between reproduction and maintenance, and in many species reproduction and senescence are closely linked. Recent studies of mice, Caenorhabditis elegans, and Drosophila suggest that there is a conserved genetic pathway that regulates aging and that it can indeed be selected for. This pathway involves the response to insulin and insulin-like growth factors, Name of these transcription factors are

#Question id: 7767

#SCPH06 I Botany

How can evolution select for phenotypes that postpone reproduction or sexual maturity?” There is often a trade-off between reproduction and maintenance, and in many species reproduction and senescence are closely linked. Recent studies of mice, Caenorhabditis elegans, and Drosophila suggest that there is a conserved genetic pathway that regulates aging and that it can indeed be selected for. This pathway involves the response to insulin and insulin-like growth factors, Name of these transcription factors are

#Question id: 7767

#SCPH28 | Zoology

How can evolution select for phenotypes that postpone reproduction or sexual maturity?” There is often a trade-off between reproduction and maintenance, and in many species reproduction and senescence are closely linked. Recent studies of mice, Caenorhabditis elegans, and Drosophila suggest that there is a conserved genetic pathway that regulates aging and that it can indeed be selected for. This pathway involves the response to insulin and insulin-like growth factors, Name of these transcription factors are

#Question id: 7768

#SCPH01 Biochemistry

The insulin signaling pathway inhibits the synthesis of the Foxo transcription factor proteins that would otherwise increase cellular longevity. Downregulation of the insulin signaling pathway has several other functions, choose right one

#Question id: 7768

#I Life Science/ Life Sciences Group – I-V

The insulin signaling pathway inhibits the synthesis of the Foxo transcription factor proteins that would otherwise increase cellular longevity. Downregulation of the insulin signaling pathway has several other functions, choose right one