#Question id: 643
#SCPH05 I Biotechnology
The hydrolysis of lactose is catalysed by b-galactosidase as given below
Which of the following could be correct mechanism for this hydrolysis reaction?
A. Nucleophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
B. Electrophilic attack by the carboxylate group of Glu/Asp on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
C. Nucleophilic attack by the carboxylate group of Lys/Arg/His or bivalent cation on the C-1 carbon atom of the galactose moiety within glucose, releasing glucose and forming an intermediate with the galactose linked to the enzyme through an ester linkage. Water then attacks this carbon atom, displacing the glutamate carboxylate and releasing galactose.
#Question id: 5051
#SCPH28 | Zoology
fter the 8-cell stage, the mammalian embryo develops an outer layer (which becomes the fetal portion of the placenta), and an inner cell mass that generates all the cells of embryo. The cells of the inner cell mass are thus said to be-
#Question id: 764
#SCPH05 I Biotechnology
Which of the following is NOT correct about tRNA?
#Question id: 27895
#Research Methodology
Integrity and transparency are fundamental:
#Question id: 11149
#SCPH28 | Zoology
The Nernst equation specifies the equilibrium potential for a particular ion. This equilibrium potential is a function of ________.