E. coli was continuously cultured in a continuous stirred tank fermenter with a working volume of 1.0 I by chemostat. A medium containing 4.0 g1^-1 of glucose as a carbon source was fed to the fermenter at a constant flow rate of 0.5 I h^-1, and the glucose concentration in the output stream was 0.20 g I^-1. The cell yield with respect to glucose YxS was 0.42 g dry cells per gram glucose. Determine specific growth rate 

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#Question id: 3510

#SCPH06 I Botany

Assume that the trihybrid cross AA BB RR X aa bb rr is made in a plant species in. What proportion of F2 progeny would be expected to be homozygous for all three genes when independently assort?

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#Question id: 15182

#SCPH28 | Zoology

Which of the following types of effect are responsible for lowering the efficiency of electrophoresis?

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#Question id: 5061

#I Life Science/ Life Sciences Group – I-V

 The activin family, the bone morphogenetic proteins (BMPs), the Nodal proteins, the Vg1 family included in-

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#Question id: 754

#SCPH01 Biochemistry

If the twisting of the DNA is in the same direction as that of the double helix, that is the helix is twisted up before closure, this form of super coiling is known as;

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#Question id: 13091

#SCPH06 I Botany

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?